The best Side of attune flow cytometer
The best Side of attune flow cytometer
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At that time, the early indicators have been dismissed as debris, although the retrospective evaluation shown shared qualities While using the problematic cells in lifestyle. Tracing the an infection aided the lab create further laboratory processes for screening and defense of assay-crucial mobile strains.
No matter if you’re a starter or a specialist, discover our assets to progress your research and know-how in flow cytometry.
(B) RAW 264.seven macrophages are frequently annotated inaccurately with regard to particle count due to their complexity and noncircular borders. The number of singlets readily available for Investigation will increase when the person trains and employs a more precise design.
Find new insights into your experimental data by visualizing populations making use of combined fluorescence and morphology knowledge. Our program interface wizards help downsampling, concatenation (merged documents) and dimensionality resources which might be crafted in the software for an intuitive, streamlined information Evaluation encounter.
*Pricing is indicative only and is topic to vary. Please Speak to your Agilent representative with any questions. The data set forth herein just isn't a suggestion or simply a motivation, each of which are subject to credit rating approval, terms and conditions, and availability with Agilent. Agilent Fiscal Alternatives is obtainable in selected international locations.
Right here, a skilled user has gated singlets confidently. After assessing the guide singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate incorporates much more than four% aggregates.
Optimizing gating method. Even sturdy handbook singlet gating is mistake-vulnerable and remains a subjective conclusion position in Nearly all flow cytometry assays. Imaging can be utilized to confirm and adjust gates to include only one cells of fascination.
Our researchers will layout panels for you personally as again and again you need—cost-free, no solution invest in needed.
The Attune NxT cytometer is the dependable workhorse instrument your lab can count on for steady and trustworthy performance. The Attune CytPix model incorporates a significant-velocity brightfield digital camera and AI-driven picture Evaluation application, with current updates like equipment Studying resources and an intuitive consumer interface, enabling Increased facts precision, precision, and more rapidly insights into sample biology.
End users can see function photographs in serious-time to confirm sample top quality, enhance protocols, and record the morphology of each mobile inhabitants determined for Examination. Imaging capture also Rewards from acoustic focusing, which really helps to posture the cells to make sure that a sharp, centered graphic get more info is acquired.
Sound waves enable precise mobile alignment, leading to Outstanding facts excellent and ~10X speedier sample flow fees.
We Formerly demonstrated the power of imaging Car or truck-T/Ramos cell interactions. Enable’s examine just the population of finest interest, the double beneficial gatherings, To find out more. We can now use prolonged morphological parameters (right here, circularity and skewness of intensity) to further look at the functions of those populations and refine gating on these events, rising data robustness.
Developed-in dimensionality reduction applications improved resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to verify anticipated populace resolution in lysed full human blood. User can look at activities from Each individual cluster for visual affirmation of mobile phenotypes.
No clean/no lyse leukocyte Examination with imaging. Cells were obtained from 24-hour-aged blood by dilution in one mM EDTA ( Expert UX/UI allows customers to teach AI versions for expanded applications. (A) The “Teach Product” feature gives annotation equipment and real-time product teaching accuracy.
At the heart of every Attune Flow Cytometer lies our innovative acoustic focusing know-how, which utilizes seem waves to meticulously align cells in the flow cell. This impressive technique surpasses standard hydrodynamic procedures, causing a lot quicker processing speeds, Excellent facts high-quality, and amplified sample throughput.
Since the mobile line had previously been analyzed to the Attune CytPix Flow Cytometer, the researchers went again to the photographs and have been ready to document the microbial an infection at least 5 days earlier.
Agilent Benefit Assure ensures you at the very least ten years of instrument use from the day you purchase. If not, We're going to credit rating you With all the residual worth of the method toward an upgraded model.
What seemed to be a homogeneous population is really additional diverse—an insight that ought to be regarded when interpreting effects.